Improved High-Performance Liquid Chromatography Method to Determine Theobromine and Caffeine in Cocoa and Cocoa Products

Pura Naik, J. (2001) Improved High-Performance Liquid Chromatography Method to Determine Theobromine and Caffeine in Cocoa and Cocoa Products. Journal of Agricultural and Food Chemistry, 49. pp. 3579-3583. ISSN 0021-8561

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Abstract

<p align="justify">At present, the commonly used HPLC method for the analysis of caffeine and theobromine contents in aqueous cocoa extracts employs direct application of the extracts on the column. This practice gradually reduces the efficiency of the column and shortens its life. Also, this method gives inflated values due to interfering substances and difficulty in achieving baseline resolution. In the improved method, the interfering cocoa pigments are effectively removed by passing the aqueous extract through a Sep-pak C₁₈ cartridge. Subsequent injection on a C₁₈ reverse-phase column employing acetonitrile and water (20:80) as the mobile phase reduces the analysis time without affecting either resolution of the peak or the accuracy of caffeine and theobromine determination or achieving baseline resolution. Therefore, this method is ideally suited for rapid routine analysis of cocoa and its products.</p>

Item Type:	Article
Uncontrolled Keywords:	Theobromine; caffeine; Cocoa; Sep-pak C<sub>18</sub>; fermented cocoa beans; HPLC
Subjects:	600 Technology > 07 Beverage Technology > 03 Cocoa
Divisions:	Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Jun 2005
Last Modified:	28 Dec 2011 09:24
URI:	http://ir.cftri.res.in/id/eprint/56

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