Eugenol—The active principle from cloves inhibits 5-lipoxygenase activity and leukotriene-C4 in human PMNL cells.

Raghavendra, H. and Diwakar, B. T. and Lokesh, B. R. and Naidu, K. A. (2006) Eugenol—The active principle from cloves inhibits 5-lipoxygenase activity and leukotriene-C4 in human PMNL cells. Prostaglandins, Leukotrienes and Essential Fatty Acids, 74 (1). pp. 23-27. ISSN 0952-3278

PDF Prostaglandins,_Leukotrienes_and_Essential_Fatty_Acids_74_(2006)_23-27.pdf Restricted to Registered users only Download (165kB)

Abstract

Polymorphonuclear leukocytes (PMNL) play an important role in the modulation of inflammatory conditions in humans. PMNL cells recruited at the site of inflammation, release inflammatory mediators such as leukotrienes, proteolytic enzymes and reactive oxygen species. Among these, leukotrienes are implicated in pathophysiology of allergic and inflammatory disorders like asthma, allergic rhinitis, arthritis, inflammatory bowel disease and psoriasis. 5-Lipoxygenase (5-LO) is the key enzyme in biosynthetic pathway of leukotrienes. Our earlier studies showed that spice phenolic active principles significantly inhibit 5-LO enzyme in human PMNLs. In this study we have further characterized the inhibitory mechanism of eugenol, the active principle of spice-clove on 5-LO enzyme and also its effect on leukotriene C₄ (LTC₄). Substrate dependent enzyme kinetics showed that the inhibitory effect of eugenol on 5-LO was of a non-competitive nature. Further, eugenol was found to significantly inhibit the formation of LTC₄ in calcium ionophore A23187 and arachidonic acid (AA) stimulated PMNL cells. These data clearly suggest that eugenol inhibits 5-LO by non-competitive mechanism and also inhibits formation of LTC₄ in human PMNL cells and thus may have beneficial role in modulating 5-LO pathway in human PMNL cells.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Elsevier Ltd.
Subjects:	600 Technology > 08 Food technology > 30 Spices/Condiments
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Apr 2007
Last Modified:	28 Dec 2011 09:27
URI:	http://ir.cftri.res.in/id/eprint/568

Actions (login required)

View Item