Preparation of protoplasts from the cyanobacterium Spirulina platensis and a novel viability assay.

Priya Sethu, K. M. and Prabha, T. N. and Venkataraman, L. V. (1994) Preparation of protoplasts from the cyanobacterium Spirulina platensis and a novel viability assay. Letters in Applied Microbiology, 18 (5). 241-244, 7 ref..

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Abstract

[Cyanobacterial genus Spirulina produces proteins, beta-carotene, vitamins (including B12), and other commercially important products, e.g. phycocyanin, gamma-linolenic acid, and superoxide dismutase. For commercial purposes, improvement of strains through genetic manipulation is desirable.] A method is described for the isolation of protoplasts from Spirulina platensis using an elite strain (FT 06) selected after screening. Trichomes of the cyanobacterium were treated with lysozyme (0.1%) for 28 h at room temp. in 0.03 mol/l phosphate buffer (pH 6.8) with 0.5 mol/l mannitol as the osmoticum. This resulted in an 80% yield of protoplasts. Cells were intact in their fine structure and viable as evidenced by a rapid and efficient viability assay using a novel mixture of fluorescein diacetate and calcofluor white.

Item Type:	Article
Uncontrolled Keywords:	ALGAE-; CELLS-; MICROORGANISMS-; PROTOPLASTS-; SPIRULINA-
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	01 May 2012 11:11
Last Modified:	01 May 2012 11:11
URI:	http://ir.cftri.res.in/id/eprint/6531

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