Gossypol removal and functional properties of protein produced by extraction of glanded cottonseed with different solvents.

Cottonseed flakes were extracted with one of the following solvents: (i) hexane; (ii) 1:1 mixture of 85% isopropanol and hexane; and (iii) acetone followed by 1:1 mixture of isopropanol and hexane. Meal (ii) had the lowest free gossypol content (0.069%). Functional properties and protein content of all meals were similar. Ultracentrifugation did not reveal any change in the proportion of the protein fractions. Gel electophoresis indicated minor differences.