Isolation of Streptomyces having high glucose-isomerase activity and assessment of their efficiency in the production of fructose syrups.

Joseph, R. and Shanthamma, M. S. and Sreenivasa Murthy, V. (1977) Isolation of Streptomyces having high glucose-isomerase activity and assessment of their efficiency in the production of fructose syrups. Journal of Food Science and Technology, India, 14 (2). 73-77, 21 ref..

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Abstract

Strains of (i) Streptomyces fradiae, SCF-5 and (ii) S. cinnamonensis, MFS-4 capable of producing glucose isomerase were isolated from farm composts and agricultural fields. These were allowed to grow in the prescribed medium at 28-30 degree C for 4 days. They produced glucose isomerase ranging from 219 to 1348 units/g dry cells. The enzyme was cell bound and its activity was max. at pH 7-8 and at 60-80 degree C. The capacities of (i) and (ii) cell suspensions to convert glucose to fructose were tested in 20 ml of the reaction mixture. Enzyme from (i) effected greater conversion of glucose to fructose at 60 degree C during 24 h than (ii). The fructose syrup after decolorization, deionization and flash evaporation yielded a clear syrup with 65-70% DM content.

Item Type:	Article
Uncontrolled Keywords:	SUGAR-SYRUPS; fructose syrups, Streptomyces glucose isomerases manufacture of; GLUCOSE-; FRUCTOSE-; Streptomyces glucose isomerases manufacture of fructose syrups; ISOMERASES-; ACTINOMYCETALES-
Subjects:	600 Technology > 08 Food technology > 25 Sugar/Starch/Confectionery
Divisions:	Sensory Science
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	23 Mar 2010 07:33
Last Modified:	23 Mar 2010 07:33
URI:	http://ir.cftri.res.in/id/eprint/7040

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