Aparna, H. S. and Salimath, P. V. (2001) Purification of an antigenic glycopeptide from buffalo colostrum. Journal of Food Science and Technology, 38 (5). 450-452, 27 ref..
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Abstract
Purification and characterization of a glycopeptide from buffalo colostrum is described. The sialoglycopeptide was fractionated and purified on Sephadex G-25 and QAE-Sephadex A-25. Its homogeneity was confirmed by RP-HPLC and electrophoresis. It consisted of NeuNAc, Fuc, Gal, Man, GlcNAc in the ratio 1:1:2:1:1 and Asp, Glu, Ser, Thr, Pro as major amino acids. Lys was found to be the N-terminal amino acid residue. Antibodies raised to the glycopeptide were immunogenic and were similar to blood group-A substance as determined by immunodouble diffusion and haemagglutination techniques.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLOSTRUM-; PEPTIDES-; PURIFICATION-; GLYCOPEPTIDES- |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry |
| Divisions: | Dept. of Biochemistry |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 01 Jun 2011 07:17 |
| Last Modified: | 28 Dec 2011 09:59 |
| URI: | http://ir.cftri.res.in/id/eprint/7624 |
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