Aqueous two phase extraction for purification and concentration of betalains

As a natural food colorant, betalains are finding increased importance. The presence of free sugars accelerates the degradation of
betalains, hence it is desirable to separate the sugars from the beet extract. Aqueous two-phase extraction was employed for the first time
as an attractive alternative for the downstream processing of betalains, mainly for the removal of sugars without the need of additional
steps. The effect of different parameters such as tie line length, phase volume ratio, concentration of phase forming components on the
partitioning behavior of betalains was studied. The differential partitioning of betalains and sugars was achieved in aqueous two phase
extraction at higher tie line length (34%), wherein betalains (about 70–75%) and sugars (about >90%) are partitioned in the top and bottom
phases, respectively. Further, the betalains was successfully separated from the polymer employing organic–aqueous extraction
resulting in 3.4 fold increase in concentration.