Organogenesis from cotyledon and hypocotyl-derived explants of japhara (Bixa orellana L.)

A protocol for direct organogenesis in Bixa orellana (pink flowers variety) has been
developed with significant organogenic response from rooted hypocotyls, hypocotyl segments,
and cotyledonary leaf explants on Murashige and Skoog (MS) medium supplemented
with 2.0 mg L–1 thidiazuron and 0.25% coconut water or 7.0 mg L–1 N6-Benzyladenine
and 0.1 mg L–1 a-naphthalene acetic acid. Thidiazuron in combination with
coconut water promoted higher organogenic response in rooted hypocotyls. Similarly direct
organogenesis was noticed from hypocotyls, cotyledonary leaf explants and shoot tip
explants on half MS medium with 0.25 mg L–1 N6-Benzyladenine (BA) and 0.5 mg L–1
indole-3-acetic acid (IAA). Best shoot elongation of shoot buds was achieved in the presence
of 1.5 mg L–1 (BA) + 1.0 mg L–1indole-3-butyric acid (IBA). The in vitro rooting of
microshoots was good in the presence of 3.0 mg L–1 (IBA). Seventy percent of rooted
plants survived after acclimatization.