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Influence of putrescine on anthocyanin production in callus cultures of Daucus carota mediated through calcium ATPase
Sudha, G. and Ravishankar, G. A. (2003) Influence of putrescine on anthocyanin production in callus cultures of Daucus carota mediated through calcium ATPase. Acta Physiologiae Plantarum, 25 (1). pp. 69-75.
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Abstract
The influence of Putrescine (Put) on the growth and elicitation of anthocyanin in callus cultures of Daucus carota var. Nantes scarlet was investigated through the use of ct-DL-difluoromethylarginine (DFMA), the polyamine (PA) biosynthetic inhibitor. It was observed that the addition of Put (0.05 mM) resulted in enhancement of growth and anthocyanin content. The anthocyanin content was found to be enhanced by 1.68 fold on the 21 st day as compared to the untreated controls. The PA inhibitor was found to result in lowering of the growth and the anthocyanin accumulation, which could be partially restored by the addition of Put in combination with this inhibitor. The levels of Ca2+ATPase were also found to be elevated in treatment with Put suggesting the involvement of calcium in the elicitation of anthocyanin. The endogenous titres of PAs and the ethylene production under these treatments were also studied. The treatment with DFMA resulted in lower levels of endogenous PAs and higher levels of ethylene. Lowering of ethylene by putrescine treatment shows that PA treatment also inhibited ethylene formation, which would also imply that endogenous ethylene does not influence anthocyanin production in carrot callus cultures.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | anthocyanin, DFMA, DFMO, polyamines, putrescine |
| Subjects: | 600 Technology > 08 Food technology > 23 Vegetables > 04 Carrot 500 Natural Sciences and Mathematics > 10 Plants > 04 Plant Physiology |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 07 Jun 2011 10:33 |
| Last Modified: | 28 Dec 2011 10:23 |
| URI: | http://ir.cftri.res.in/id/eprint/10083 |
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