Influence Of Phenylacetic Acid On Clonal Propagation Of Decalepis Hamiltonii Wight & Arn: An Endangered Shrub

Giridhar, P. and Vijaya Ramu, D. and Obul Reddy, B. and Rajasekaran, T. and Ravishankar, G. A. (2003) Influence Of Phenylacetic Acid On Clonal Propagation Of Decalepis Hamiltonii Wight & Arn: An Endangered Shrub. In Vitro Cellular and Developmental Biology - Plant , 39. pp. 463-467.

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Abstract

We have developed a highly efficient two-stage protocol for induction of multiple shoots from single node in vitro shoot tip explants of Decalepis hamiltonii. It was found that phenylacetic acid (PAA) had a synergistic effect on shoot multiplication when treated with N6-benzyladenine (BA). This protocol used PAA for both multiple shoot induction from nodal explants, elongation of primary shoots, and initiation of adventitious shoot formation from primary shoots. Murashige and Skoog medium containing BA (2.22–31.08mM) and a-naphthaleneacetic acid (0.27–10.74mM) or PAA (7.34–36.71mM) was used to initiate shoot formation from nodal explants. The maximum number of shoots per culture was produced on a medium containing 31.08mM BA and 14.68mM PAA, while the longest shoot length and nodes were obtained on medium containing 22.2mM BA and 14.68mM PAA. Shoots subcultured on MS medium containing 22.2mM BA and 14.68mM PAA elongated along with secondary shoot formation. The shoots were rooted on medium containing 9.8mM indole-3-butyric acid. The plantlets were acclimatized in soil with an 80–90% survival rate under field conditions.

Item Type:	Article
Uncontrolled Keywords:	axillary bud; explant; multiple shoots; plantlets; rooting.
Subjects:	500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Jun 2011 10:38
Last Modified:	28 Dec 2011 10:23
URI:	http://ir.cftri.res.in/id/eprint/10084

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