Stimulation of glucose uptake by Musa sp. (cv. elakki bale) flower and pseudostem extracts in Ehrlich ascites tumor cells

Jamuna, J. Bhaskar and Salimath, P. V. and Nandini, Chilkunda D. (2011) Stimulation of glucose uptake by Musa sp. (cv. elakki bale) flower and pseudostem extracts in Ehrlich ascites tumor cells. Journal of the Science of Food and Agriculture, 91. pp. 1482-1487.

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Abstract

BACKGROUND: Glucose uptake study plays a major role in diabetes research. Impaired glucose uptake has been implicated in the development of hyperglycemia during diabetes. Banana plant is known for its anti-diabetic properties and our earlier report revealed that banana flower and pseudostem of Musa sp. cv. elakki bale is beneficial during diabetes in rat models. The present study was designed to evaluate the potential effect of banana flower and pseudostem extracts on glucose uptake in Ehrlich ascites tumor (EAT) cells using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG), a fluorescent analogue of 2-deoxyglucose. RESULTS: Methanol and aqueous extracts of banana flower and pseudostem were more potent in promoting glucose uptake in EAT cells, in comparison to acetone and ethanol extracts. At 20 μg dosage, highest net glucose uptake was observed in aqueous extracts of banana flower (18.17±0.43 nmol L−1) and pseudostem (19.69±0.41 nmol L−1). Total polyphenol content was higher in methanol (9.031 ± 0.036 g kg−1) and aqueous (6.862 ± 0.024 g kg−1) extracts of banana flower compared to pseudostem, which were 0.442 ± 0.006 and 0.811 ± 0.011 g kg−1, respectively. CONCLUSION: Banana flower and pseudostem extracts are able to promote glucose uptake into the cells, presumably through glucose transporters 1 and 3,which could be beneficial in diabetes. Glucose uptake is likely promoted by phenolic acids besides other bioactives. It can be hypothesized that consumption of nutraceutical-rich extract of banana flower and pseudostem could replace some amount of insulin being taken for diabetes. �c 2011 Society of Chemical Industry

Item Type:	Article
Uncontrolled Keywords:	glucose uptake; diabetes; EAT cells; banana flower; banana pseudostem; extracts
Subjects:	600 Technology > 01 Medical sciences > 04 Diabetes Mellitus 600 Technology > 08 Food technology > 24 Fruits > 02 Banana
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	21 Jun 2011 11:06
Last Modified:	14 Dec 2016 10:27
URI:	http://ir.cftri.res.in/id/eprint/10158

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