An immobilized dehydrohalogenase based potentiometric biosensor for the detection of chlorinated pesticides

Murthy, Mahadevu Rajashekara and Mandappa, I. M. and Latha, R. and Vinayaka, A. C. and Thakur, M. S. and Manonmani, H. K. (2010) An immobilized dehydrohalogenase based potentiometric biosensor for the detection of chlorinated pesticides. Analytical Methods, 2 . pp. 1355-1359.

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Abstract

Among the organochlorine pesticides, DDT and HCH are the major cause for food and environmental contamination. To detect them effectively in water samples an immobilized dehydrohalogenase based potentiometric biosensor was developed. The chloride ion released as a result of dehalogenation by immobilized dehydrohalogenase during the degradation of DDT and HCH, respectively, was detected using an ion selective electrode. The voltage response had a direct linear relationship with the concentration of chloride (Cl�) released by DDT and HCH, respectively. The immobilization was advantageous in improving the enzyme property and could be used repeatedly without losing activity.

Item Type:	Article
Uncontrolled Keywords:	DDT, organochlorine pesticides, immobilization
Subjects:	600 Technology > 05 Chemical engineering > 06 Appropriate Technology 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
Divisions:	Fermentation Technology and Bioengineering Food Safety Analytical Quality Control Lab
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	20 Jul 2011 11:26
Last Modified:	22 Dec 2016 11:35
URI:	http://ir.cftri.res.in/id/eprint/10287

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