Separation and Purification of Lipase using Reverse Micellar Extraction: Optimization of Conditions by Response Surface Methodology

Nandini, K. E. and Rastogi, Navin K. (2010) Separation and Purification of Lipase using Reverse Micellar Extraction: Optimization of Conditions by Response Surface Methodology. Biotechnology and Bioprocess Engineering , 15. 349-358 .

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Abstract

Downstream processing of lipase involving reverse micellar extraction of lipase using cationic surfactant cetyltrimethylammonium bromide (CTAB) was investigated. Effect of various process parameters on both forward and backward extraction of lipase from crude extract was studied to optimize its yield and purity. Complex interaction of salt concentration (0.05~0.15M), surfactant concentration (0.10~0.30 M), and pH (6.0~9.0) for forward extraction, as well as, salt concentration (0.5~1.5 M) and pH (6.0~9.0) for backward extraction have been studied using response surface methodology. Optimum processing conditions, namely, salt concentration 0.16M, surfactant concentration 0.20 M, and pH 9.0 for forward extraction, as well as, salt concentration 0.80 M and pH 7.23 for backward extraction, fulfill the conditions to obtain activity recovery of lipase ≥78% and purification factor of lipase ≥4.0. The study demonstrated that response surface methodology can be used for optimization of the conditions for reverse micellar extraction of lipase.

Item Type:	Article
Uncontrolled Keywords:	reverse micellar extraction, downstream processing, lipase, cationic surfactant, activity recovery, response surface methodology
Subjects:	600 Technology > 08 Food technology > 05 Processing and Engineering 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions:	Food Engineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	20 Jul 2011 11:35
Last Modified:	28 Dec 2011 10:26
URI:	http://ir.cftri.res.in/id/eprint/10289

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