Purification and Characterization of Carboxylesterase from the Rat Testis

Venkatesh, S. (2001) Purification and Characterization of Carboxylesterase from the Rat Testis. Masters thesis, University of Mysore.

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type:	Thesis (Masters)
Additional Information:	<p align="justify">The mammalian testicular tissue contains very high levels of CaE somewhat equal to or exceeding that of liver, the significance of which is not clear at present. It is conceivable that CaEs being the well known molecular targets for several xenobiotics including OP pesticides, could be involved in protecting the germinal tissue from environmental toxicants which could impair spermatogenesis or produce damage that could to lead to genetic effects. Treatment of rats with the selective Leydig cell toxicant ethane dimethane sulfonate eliminated the expression of CaE in the testis. CaEs may be physiologically important since testicular dysfunction in rats treated with tri-orthotosyl phosphate (TOTP) affects spermatogenesis whereas Leydig cells appear to be protected from the toxic effects of TOTP possibly due to the presence of high levels of carboxylesterases. Purification and characterization of the testicular CaE is essential in order to understand the biochemical role and physiological significance of CaE in the ; reproductive system. Although carboxylesterases have been purified and characterized from a variety of tissues like brain, liver, kidney and lung, there is a dearth of information on the testis carboxylesterase. The objective of the present investigation is to purify and characterize an OP- sensitive carboxyl esterase from the testis of rats with a view to understand the role of carboxylesterase in testicular detoxication mechanisms. Further ontogenic change of CaE activity and isozymal variation during postnatal development of rat testis has been studied.</p>
Uncontrolled Keywords:	rat testis carboxylesterase CaE genetic effects
Subjects:	600 Technology > 01 Medical sciences > 17 Toxicology 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry 500 Natural Sciences and Mathematics > 11 Animals
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	28 Jun 2005
Last Modified:	28 Dec 2011 09:24
URI:	http://ir.cftri.res.in/id/eprint/104

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