Interaction of sesamol (3,4-methylenedioxyphenol) with tyrosinase and its effect on melanin synthesis.

Mahendra Kumar, C. and Sathisha, U. V. and Shylaja, Dharmesh and Appu Rao, A. G. (2011) Interaction of sesamol (3,4-methylenedioxyphenol) with tyrosinase and its effect on melanin synthesis. Biochimie, 93. pp. 562-569. ISSN 0300-9084

PDF Biochimie, Volume 93, Issue 3, March 2011, Pages 562-569.pdf - Published Version Restricted to Registered users only Download (929kB) | Request a copy

Abstract

Sesamin, sesamolin (lignans) and sesamol e from sesame seed (Sesamum indicum L.) e are known for their health promoting properties. We examined the inhibition effect of sesamol, a phenolic degradation product of sesamolin, on the key enzyme in melanin synthesis, viz. tyrosinase, in vitro. Sesamol inhibits both diphenolase and monophenolase activities with midpoint concentrations of 1.9 mM and 3.2 mM, respectively. It is a competitive inhibitor of diphenolase activity with a Ki of 0.57 mM and a noncompetitive inhibitor of monophenolase activity with a Ki of 1.4 mM. Sesamol inhibits melanin synthesis in mouse melanoma B16F10 cells in a concentration dependant manner with 63% decrease in cells exposed to 100 mg/mL sesamol. Apoptosis is induced by sesamol, limiting proliferation. This study of the chemistry and biology of lignans, in relation to the mode of action of bioactive components, may open the door for drug applications targeting enzymes.

Item Type:	Article
Uncontrolled Keywords:	Sesamol, Diphenolase, Mushroom tyrosinase Cell lines, Melanin, Hyperpigmentation
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions:	Dept. of Biochemistry Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Feb 2012 04:08
Last Modified:	15 Jan 2021 06:01
URI:	http://ir.cftri.res.in/id/eprint/10592

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