![[feed]](/style/images/feed-icon-14x14.png){kind=icon}
Combined Effect of Enterocin and Lipase From Enterococcus faecium NCIM5363 Against Food Borne Pathogens: Mode of Action Studies.
Vrinda, R. and Bhaskar, N. and Prakash, M. Halami (2012) Combined Effect of Enterocin and Lipase From Enterococcus faecium NCIM5363 Against Food Borne Pathogens: Mode of Action Studies. Current Microbiology, 65. pp. 162-169.
![[img]](http://ir.cftri.res.in/style/images/fileicons/application_pdf.png) |
PDF
Current Microbiology, 2012, Volume 65, Number 2, Pages 162-169.pdf - Published Version Restricted to Registered users only Download (602kB) |
Abstract
Food borne diseases have a major impact on public health whose epidemiology is rapidly changing. The whole cells of pathogens involved or their toxins/metabolites affect the human health apart from spoiling sensory properties of the food products finally affecting the food industry as well as consumer health. With pathogens developing mechanisms of antibiotic resistance, there has been an increased need to replace antibiotics as well as chemical additives with naturally occurring bacteriocins. Bacteriocins are known to act mainly against Gram-positive pathogens and with little or no effect towards Gramnegative enteric bacteria. In the present study, combination effect of lipase and bacteriocin produced by Enterococcus faecium NCIM5363, a highly lipolytic lactic acid bacterium against various food pathogens was assessed. The lipase in combination with enterocin exhibited a lethal effect against Gram-negative pathogens. Scanning electron microscopy studies carried out to ascertain the constitutive mode of action of lipase and enterocin revealed that the lipase degrades the cell wall of Gram-negative bacteria and creates a pore through which enterocin enters thereby resulting in cell death. The novelty of this work is the fact that this is the first report revealing the synergistic effect of lipase with enterocin against Gram-negative bacteria.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Food borne diseases, Enterococcus faecium, lipase |
| Subjects: | 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 09 Food Microbiology |
| Divisions: | Food Microbiology Meat Fish and Poultry Technology |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 27 Aug 2012 06:24 |
| Last Modified: | 27 Aug 2012 06:24 |
| URI: | http://ir.cftri.res.in/id/eprint/10959 |
Actions (login required)
 |
View Item |