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Downstream processing of luciferase from fireflies (Photinus pyralis) using aqueous two-phase extraction.

Priyanka, B. S. and Rastogi, N. K. and Raghavarao, K.S.M.S. and Thakur, M. S. (2012) Downstream processing of luciferase from fireflies (Photinus pyralis) using aqueous two-phase extraction. Process Biochemistry, 47 . pp. 1358-1363. ISSN 1359-5113

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Abstract

The firefly luciferase has been extensively used for sensitive detection of bacteria, gene expression and environmental toxins (biosensors). The aim of the present study was to design a simple and more efficient method for the purification and concentration of luciferase using aqueous two-phase extraction (ATPE). Downstream processing of luciferase from North American Firefly Photinus pyralis was carried out, for the first time, using polymer/salt aqueous two phase system (ATPS) at 4 ◦C. The enzyme was observed to preferentially partition to the polyethylene glycol (PEG) rich top phase. The best results of purification (13.69 fold) and enzyme activity recovery (118.34%) were observed in the system containing 4.0% (w/w) PEG (1500) and 20.5% (w/w) (NH4)2SO4 with a phase volume ratio of 0.21.

Item Type: Article
Uncontrolled Keywords: Downstream processing Luciferase, Bioseparation, Aqueous two-phase extraction Purification
Subjects: 600 Technology > 08 Food technology > 07 Food Engineering
Divisions: Fermentation Technology and Bioengineering
Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 Aug 2012 06:44
Last Modified: 29 Dec 2016 13:11
URI: http://ir.cftri.res.in/id/eprint/10981

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