Evaluation of Various Culture Conditions for the Production of Poly Unsaturated Fatty Acids (PUFAs) from selected Marine Micro-Algae.

Parul, Yadav (2015) Evaluation of Various Culture Conditions for the Production of Poly Unsaturated Fatty Acids (PUFAs) from selected Marine Micro-Algae. [Student Project Report] (Submitted)

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Abstract

Polyunsaturated fatty acids (PUFAs) have been associated with various health benefits and are commonly obtained from fish oil. However continuous exploitation of fish biodiversity for PUFA rich oil would create ecological imbalance. Hence identifying alternative sources for production of PUFA rich oil is necessary. Marine microalgae can be a targeted as vegetarian source of PUFAs since they are primary producers of aquatic ecosystem and marine organisms like fish feed on microalgae for meeting their nutritional requirements. Hence towards this direction, two marine microalgal species belonging to the genus of Tetraselmis sp. (Tetraselmistheli and Tetraselmischuii) were evaluated as source of omega 3 PUFA mainly Eicosapentaenoic acid (C-20:5). The selected microalgal strains were maintained at CSIR-CFTRI algal culture facility. The strains were evaluated for their growth, biomass accumulation and lipid profile under different cultivation parameters such as light intensity and photo-period, carbon supplementation (sodium acetate), incubation temperature and salinity levels. High light intensity at 60 μE m−2 s−1 increased growth and biomass concentration and lipid content in both the strains, however the EPA content decreased by 2 folds at higher light intensity. Under colder incubation conditions (15°C) the total lipid content EPA content increased by 1.5 to 2 folds, without affecting the biomass concentration. Maximum growth and biomass concentration was observed at 25mM acetate supplementation for both the strains. However with increasing acetate levels the total lipid and EPA content decreased by 2-3 folds. Both the strains could tolerate high salinity (1M NaCl), however showed a reduced growth and biomass concentration. The monounsaturated fatty acid levels such as palmito-oleic acid (C-16:1) and oleic acid (C-18:1) increased with increasing salinity, while EPA content did not show any significant variations. From this study it is evident that the Tetraselmis sp. could tolerate stressful culture conditions such as colder incubation temperature and high salt concentration and could be exploited as potential source of EPA for nutritional applications.

Item Type:	Student Project Report
Uncontrolled Keywords:	Polyunsaturated fatty acids, fish oil, Marine microalgae, Tetraselmis sp
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	06 Nov 2015 07:48
Last Modified:	06 Nov 2015 07:48
URI:	http://ir.cftri.res.in/id/eprint/11941

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