Carotenoid Profile of Edible Japanese Seaweeds: An Improved HPLC Method for Separation of Major Carotenoids

Terasaki, M. and Bhaskar, N. (2012) Carotenoid Profile of Edible Japanese Seaweeds: An Improved HPLC Method for Separation of Major Carotenoids. Journal of Aquatic Food Product Technology, 21. pp. 468-479.

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Abstract

An improved multi-step gradient reverse phase high performance liquid chromatography (HPLC) method to simultaneously separate major carotenoids from natural as well as food samples was developed. Quantitative profiling of carotenoid compounds was carried out on three edible brown seaweeds (Sargassum horneri, Cystoseira hakodatensis, and Undaria pinnatifida) and three red seaweeds (Gracilaria vermiculophylla, Grateloupia asiatica, and Grateloupia livida). Fucoxanthin (Fx) was detected in all the brown seaweeds with quantities (mg g−1 dry weight [dwt]) ranging from 1.3 ± 0.3 in C. hakodatensis to 2.4 ± 0.1 in S. horneri. U. pinnatifida, commonly known as wakame, had a Fx content of 2.3 ± 0.1 mg g−1 dwt. In the case of red seaweeds, zeaxanthin (Zx) was the major carotenoid, and G. vermiculophylla had the highest Zx content (80.2 μg g−1 dwt) among the red seaweeds apart from small amounts of Fx (9.1 μg g−1 dwt). Similarly, the other two species of red seaweeds, G. asiatica and G. livida, contained (μg g−1 dwt) lutein (Lut), Fx, and Zx as the major carotenoids (G. asiatica: Lut 10.4, Fx 1.5, Zx 1.1; G. livida: Lut 9.3, Fx 3.5, Zx 1.0). The results suggest the usefulness of edible varieties (barring wakame) of seaweeds as dietary sources of carotenoids.

Item Type:	Article
Uncontrolled Keywords:	brown seaweeds, red seaweeds, carotenoid profile, HPLC, fucoxanthin, lutein, zeaxanthin
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae
Divisions:	Meat Fish and Poultry Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Sep 2016 07:29
Last Modified:	22 Sep 2016 07:29
URI:	http://ir.cftri.res.in/id/eprint/12294

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