A Spectroscopic Study of the Interaction of Nigerloxin, a Fungal Metabolite, with Serum Albumin

Sekhar Rao, K. C. and Appu Rao, A. G. and Sattur, A. P. (2004) A Spectroscopic Study of the Interaction of Nigerloxin, a Fungal Metabolite, with Serum Albumin. Lipids, 39 (2). pp. 173-177.

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Abstract

Nigerloxin [2-amido-3-hydroxy-6-methoxy-5-tethyl-4-(prop-1'-enyl) benzoic acid], a fungal metabolite, is an inhibitor of lipoxygenase and aldose reductase with free radical-scavenging properties. The interaction of nigerloxin with avine serum albumin (BSA) was investigated using fluorescence spectroscopy and circular dichroic measurements. The fuorescence of BSA was quenched following interaction with nigerloxin, and this property was used to generate a binding constant. The estimated association constant was 1.01± 0.2 x 06 M-1. Job's method of continuous variation indicated that nigerloxin formed a 1:1 ±t 0.1 complex with BSA. To understand the nature of the interaction, the variance in the association constant as a function of temperature in the range of 14-45°C was used to calculate the thermodynamic parameters. The thermodynamic parameters at 27°C derived from the mass action lot and van't Hoff's plot were as follows: ∆G = -8.2t±0.1. kcal/mol, ∆H = 0 kcal/mol, and ∆S = 27.5 ±t 0.4 cal/mol/K where ∆G is free energy, ∆H is enthalpy, and ∆S is entropy). Increasing ionic strength did not favor interaction. Circular dichroic measurements revealed that the interaction of nigerloxin with BSA did not lead to changes in the secondary structure of the protein. The reversibility of the interaction verified by the dilution method was found to be reversible. These measurements suggest that partial hydrophobic and partial ionic bonding play a role in the interaction of nigerloxin with BSA.

Item Type:	Article
Uncontrolled Keywords:	Nigerloxin fungal metabolite serum albumin
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions:	Fermentation Technology and Bioengineering Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	22 Jun 2007 09:01
Last Modified:	03 Jul 2015 08:05
URI:	http://ir.cftri.res.in/id/eprint/1242

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