Enzymatic degradation of chitosan and production of D-glucosamine by solid substrate fermentation of exo-b-D-glucosaminidase (exochitosanase) by Penicillium decumbens CFRNT15

Nidheesh, T. and Gaurav Kumar, Pal and Suresh, P. V. (2015) Enzymatic degradation of chitosan and production of D-glucosamine by solid substrate fermentation of exo-b-D-glucosaminidase (exochitosanase) by Penicillium decumbens CFRNT15. International Biodeterioration and Biodegradation, 97. pp. 97-106.

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Abstract

Exo-b-D-glucosaminidase is an enzyme acting on b-(1/4) linkage of chitosan from its non-reducing end and produces D-glucosamine as the exclusive end product. Solid substrate fermentation (SSF) production of exo-b-D-glucosaminidase by a novel isolate of Penicillium decumbens (CFRNT15) was optimized employing Box-Behnken design and response surface methodology. The optimized conditions for maximal production of exo-b-D-glucosaminidase were 22 ± 1 �C temperature, 75% (w/w) moisture, 120 h fermentation, 5.0% (w/w) chitosan, 0.05% (w/w) CaCl2, 0.5% (w/w) MgSO4, and 15.1% (w/v) inoculum. The close agreement of experiential results with the model prediction showed that the model was precise and dependable for predicting the exo-b-D-glucosaminidase production. Statistical optimization resulted in an overall increase of exo-b-D-glucosaminidase activity from 116.7 ± 4.6 to 2335.1 ± 12.4 unit/g initial dry substrate. Crude exo-b-D-glucosaminidase was found to have optimum activity at 42 ± 1 �C and pH 5.6. Crude exo-b-D-glucosaminidase produced the maximum D-glucosamine yield of 410e546 mM from different a-chitosan substrates hydrolysis. Thin layer chromatography analysis revealed that D-glucosamine was the sole end product of the chitosan hydrolysis. These results indicated the potential of P. decumbens for the production of the exo-b-D-glucosaminidase employing the cost-effective SSF process and the significance of the exo-b-D-glucosaminidase for the preparation of commercially important Dglucosamine and to cope seafood processing chitinous biomaterials.

Item Type:	Article
Uncontrolled Keywords:	Exo-b-D-glucosaminidase Solid substrate fermentation Penicillium decumbens D-glucosamine Box-Behnken design Response surface methodology
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Meat Fish and Poultry Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	28 Nov 2016 07:58
Last Modified:	28 Nov 2016 07:58
URI:	http://ir.cftri.res.in/id/eprint/12575

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