Protease Production by Aspergillus oryzae in Solid-State Fennentation Utilizing Coffee By-Products.

Pushpa, S. Murthy and Madhava Naidu, M. (2010) Protease Production by Aspergillus oryzae in Solid-State Fennentation Utilizing Coffee By-Products. World Applied Sciences Journal, 18 (2). pp. 199-205.

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Abstract

Aspergillus oryzae CFR305 was isolated from coffee residues and studied for protease production using coffee substrates lUlder solid-state fermentation. Coffee cherry husk was fOWld to be suitable substrate for protease production. The influence of process parameters such as temperature, pH, moisture, particle size, inoculum size, additives and pretreatments of substrates were evaluated. Maximum protease production of 12236 U/gds was obtained on pretreated coffee cherry husk by steam. The protease was partially purified and characterized. The enzyme was purified 33.76 fold by ammonium sulphate precipitation and ion exchange chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicates that the purified protease is a monomeric enzyme with a molecular mass of ~35 kDa. The enzyme is a serine protease as indicated by its inhibition studies. The protease enzyme showed maximum activity at pH 10 at 60°C. Thus, the alkaline serine protease indicate their use as additives in industry and also states the importance of SSF for the production of protease using coffee cherry husk as substrate, which offer significance benefit due to abundant availability and economy. This is the first report on production of protease using coffee by-products.

Item Type:	Article
Uncontrolled Keywords:	Aspergillus oryzae CFR305 . Solid-state fermentation . Coffee by-products . Protease . Pre-treatment
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 600 Technology > 07 Beverage Technology > 04 Coffee 600 Technology > 08 Food technology > 16 Nutritive value > 07 Waste utilization
Divisions:	Plantation Products Spices and Flavour Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	10 Jul 2017 09:06
Last Modified:	16 Feb 2018 04:44
URI:	http://ir.cftri.res.in/id/eprint/12782

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