Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.

Kannan, Rangiah and Palakodeti, Dasaradhi (2018) Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method. In: Planarian Regeneration: Methods and Protocols, Methods in Molecular Biology. Springer, pp. 555-570.

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Abstract

Freshwater planarian species S. mediterranea is an emerging stem cell model because of its capability of regenerating large portions of missing body parts. It is one of the best model systems available to address the basic biological mechanisms in the regeneration processes. Absolute quantification of metabolites from planarians is imperative to understand their role in the regeneration processes. Here we describe a stable isotope dilution ultrahigh performance liquid chromatography/mass spectrometry/selected reaction monitoring (UHPLC-MS/SRM) assay for a sensitive and quantitative assessment of neurotransmitters (NTs) in planaria. We used this method for the simultaneous quantification of 16 NTs from both intact and regenerating planarians.

Item Type:	Book Section
Uncontrolled Keywords:	Neurotransmitters, Quantification, Mass spectrometry, Planaria, Regeneration
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry > Fish
Divisions:	Food Safety Analytical Quality Control Lab
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	27 Aug 2018 10:30
Last Modified:	27 Aug 2018 10:30
URI:	http://ir.cftri.res.in/id/eprint/13817

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