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Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum.

Rochak, M. and Richa, Sharma and Raghavarao, K. S. M. S. (2019) Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum. Bioresource Technology, 280. pp. 277-286. ISSN 0960-8524

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Aqueous two-phase extraction (ATPE) of R-Phycoerythrin (R-PE), a fluorescent and commercially valuable protein, was carried out in two parallel schemes. In scheme-1, ATPE alone was employed, and in scheme-2, process-integration was attempted, where primary extract was subjected to precipitation before standardization of process parameters of ATPE. Scheme-1 with polyethylene glycol (PEG)-3350/potassium phosphate system has resulted in very low R-PE yield (26% w/w) without much enrichment in purity (0.3). In scheme-2, PEG-1450/potassium phosphate system (pH 6, tie-line length 12.26% and lower volume-ratio) has resulted in higher R-PE purity (0.74) with 72% yield. R-PE containing PEG-rich (top) phase was subjected to ultrafiltration, to remove phase forming components, resulting in further increase in R-PE purity (1.1). Overall enrichment in R-PE purity of 11-fold with 57% (w/w) yield and removal of about 95% of total sugars was achieved in scheme-2. Intactness of R-PE after processing was confirmed by absorbance and emission spectrum analysis.

Item Type: Article
Uncontrolled Keywords: Phycobiliproteins Seaweed Purification Ultrafiltration Downstream processing Integration
Subjects: 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae
600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Mar 2019 11:08
Last Modified: 12 Mar 2019 11:15
URI: http://ir.cftri.res.in/id/eprint/13925

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