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Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum.
Rochak, M. and Richa, Sharma and Raghavarao, K. S. M. S. (2019) Aqueous two-phase extraction of R-Phycoerythrin from marine macro-algae, Gelidium pusillum. Bioresource Technology, 280. pp. 277-286. ISSN 0960-8524
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Bioresource Technology Volume 280, May 2019, Pages 277-286.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy |
Abstract
Aqueous two-phase extraction (ATPE) of R-Phycoerythrin (R-PE), a fluorescent and commercially valuable protein, was carried out in two parallel schemes. In scheme-1, ATPE alone was employed, and in scheme-2, process-integration was attempted, where primary extract was subjected to precipitation before standardization of process parameters of ATPE. Scheme-1 with polyethylene glycol (PEG)-3350/potassium phosphate system has resulted in very low R-PE yield (26% w/w) without much enrichment in purity (0.3). In scheme-2, PEG-1450/potassium phosphate system (pH 6, tie-line length 12.26% and lower volume-ratio) has resulted in higher R-PE purity (0.74) with 72% yield. R-PE containing PEG-rich (top) phase was subjected to ultrafiltration, to remove phase forming components, resulting in further increase in R-PE purity (1.1). Overall enrichment in R-PE purity of 11-fold with 57% (w/w) yield and removal of about 95% of total sugars was achieved in scheme-2. Intactness of R-PE after processing was confirmed by absorbance and emission spectrum analysis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Phycobiliproteins Seaweed Purification Ultrafiltration Downstream processing Integration |
| Subjects: | 500 Natural Sciences and Mathematics > 08 Botanical sciences > 01 Botany > 01 Algae 600 Technology > 08 Food technology > 05 Processing and Engineering |
| Divisions: | Food Engineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 12 Mar 2019 11:08 |
| Last Modified: | 12 Mar 2019 11:15 |
| URI: | http://ir.cftri.res.in/id/eprint/13925 |
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