Cyanidin and cyanidin‐3‐glucoside derived from Vigna unguiculata act as noncompetitive inhibitors of pancreatic lipase.

Vijayaraj, P. and Nakagawa, H. and Yamaki, K. (2019) Cyanidin and cyanidin‐3‐glucoside derived from Vigna unguiculata act as noncompetitive inhibitors of pancreatic lipase. Journal of Food Biochemistry, 43. ISSN 1745-4514

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Abstract

The consumption of legumes positively correlated with the reduction of body weight. In the present study, we identified and evaluated pancreatic lipase inhibitors from Vigna unguiculata and unraveled their mode of inhibition. The highly sensitive fluoro‐ metric method was adopted to access the pancreatic lipase activity and the ethanolic extract of Vigna unguiculata showed the maximum inhibition (IC50 of 15.2 µg/ml). Cyanidin and cyanidin‐3‐glucoside are the major anthocyanins observed in Vigna unguiculata. The IC50 value of cyanidin was 28.29 µM which was 6.5‐fold higher than the cyanidin‐3‐glucoside (188.28 µM). We determined an apparent Ki of 27.28 µM for cyanidin and cyanidin‐3‐glucoside (88.97 µM) with noncompetitive inhibition. Collectively, these results suggest that the glycosylation of the anthocyanidins sig‐ nificantly reduces lipase inhibition. The noncompetitive inhibition of pancreatic li‐ pase by Vigna unguiculata anthocyanins may exert significant pharmacological activities toward obesity complications by calorie restriction. Practical applications The results of this study emphasize the importance of legumes in our diet to combat obesity‐related complications. Consumption of legumes minimizes fat absorption by inhibiting the action of the fat‐digesting enzyme.

Item Type:	Article
Uncontrolled Keywords:	anthocyanins, fats, noncompetitive inhibition, Obesity, pancreatic lipase inhibition
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions:	Dept. of Lipid Science
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	07 Oct 2020 11:29
Last Modified:	07 Oct 2020 11:29
URI:	http://ir.cftri.res.in/id/eprint/14520

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