Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice.

Aranha, Ivan and Venkatesh, Y. P. (2020) Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice. Journal of Ayurveda and Integrative Medicine, 11. pp. 140-146.

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Abstract

Background: In traditional medicine, guduchi (Tinospora cordifolia) is considered as an adaptogen with immunomodulatory prowess. A 25 kDa protein from guduchi stem has been characterized as an immunomodulatory protein (ImP). Objectives: The aim of this study was to evaluate the intrinsic immunogenicity of guduchi ImP and adjuvant activity using ovalbumin (OVA) as antigen in BALB/c mice. Materials and Methods: Mice were given guduchi ImP (30 and 60 mg) by intranasal administration to respective groups (n ¼ 6) on days 1, 14 and thereafter weekly till day 42. Immunogenic response was monitored by serum IgG/IgA levels (days 14, 35 and 50). The adjuvant activity was measured by serum anti-OVA IgG/IgA responses to administration of 30 mg OVA with guduchi ImP. The effect of guduchi ImP on the spleen status was examined by splenic weight (day 50). Results: Guduchi ImP administration displayed a significant increase in anti-guduchi ImP IgG (5e7 fold) and anti-guduchi ImP IgA (3e4 fold) on day 50 vs. control. Guduchi ImP showed a significant increase in anti-OVA IgG (6e7 fold) and anti-OVA IgA (4e5 fold) on day 50 vs. control. The splenic index of guduchi ImP group increased significantly in both the immune and adjuvant response groups; however, the splenic index in the adjuvant response group was markedly higher. Conclusion: The results indicate that guduchi ImP is a strong immunogen by itself and enhances the immunogenicity of mucosally-administered antigen in BALB/c mice. Based on the results of this animal study, it appears that guduchi ImP shows a potential for future studies in humans.

Item Type:	Article
Uncontrolled Keywords:	Adjuvant activity Guduchi Immunogenic response Immunomodulatory protein Splenic index Tinospora cordifolia
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	27 Nov 2020 10:54
Last Modified:	27 Nov 2020 10:54
URI:	http://ir.cftri.res.in/id/eprint/14668

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