Lipase-catalyzed synthesis of isoamyl isobutyrate — optimization using a central composite rotatable design

Hari Krishna, S. and Sattur, A. P. and Karanth, N. G. (2001) Lipase-catalyzed synthesis of isoamyl isobutyrate — optimization using a central composite rotatable design. Process Biochemistry, 37. pp. 9-16.

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Abstract

Rhizomucor miehei lipase, Lipozyme IM-20, was used to catalyze the esterification reaction between isobutyric acid and isoamyl alcohol to synthesize isoamyl isobutyrate in n-hexane. Response surface methodology (RSM) based on a five-level, four-variable central composite rotatable design (CCRD) was employed for optimization with respect to four important reaction variables —enzyme/substrate (E/S) ratio, substrate concentration, reaction time, and temperature. Conversion was efficient in an acid concentration range of 0.5–2.5 M, even when a very low E/S ratio (5 g/mol) was employed. Although an increase in temperature resulted in improved reaction rates, overall conversions remained practically unchanged, as the yields obtained at lower temperatures (30–40°C) were higher. Reaction time also exhibited a less significant effect on ester yields at higher E/S ratios. The optimum conditions predicted for achieving maximum ester yield (2.2 M) are: E/S ratio, 19.6 g/mol; substrate concentration, 2.5 M; reaction time, 18 h and temperature, 26.5°C.

Item Type:	Article
Uncontrolled Keywords:	Central composite rotatable design (CCRD); Enzymatic synthesis; Esterification; Isoamyl isobutyrate; Lipozyme IM-20; Response surface methodology (RSM); Rhizomucor miehei lipase
Subjects:	600 Technology > 05 Chemical engineering > 04 Fermentation Technology 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	03 Oct 2007 11:44
Last Modified:	03 Jul 2015 08:06
URI:	http://ir.cftri.res.in/id/eprint/1469

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