Sequence and N‑glycan diversity analysis of immunoglobulin G from buffalo milk using RP‑UHPLC MS/MS.

Jinesh, P. and Lijina, P. and Gnanesh Kumar, B. S. (2021) Sequence and N‑glycan diversity analysis of immunoglobulin G from buffalo milk using RP‑UHPLC MS/MS. Amino Acids.

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Abstract

Immunoglobulin G is the abundant antibody present in the colostrum and milk of major dairy animals. In the present study, bufalo milk IgG was characterized for its amino acid sequence and glycan diversity using reverse phase liquid chromatography coupled to ESI-Q-TOF MS in tandem mode. Amino acid sequence analysis of heavy chain constant region revealed the presence of two IgG subtypes namely IgG1 and IgG3, with IgG1 being the abundant. The complete light chain constant region sequence was also determined. N-glycan sequence analysis at a highly conserved site Asn–Ser–Thr revealed the presence of mainly biantennary complex type with core fucosylation (34%), bisecting GlcNAc (19%) and sialylation with both Neu5Ac and Neu5Gc (14%). The observed glycan diversity in bufalo milk IgG is in part comparable with bovine colostrum as well as human, bovine, goat serum counterparts.

Item Type:	Article
Uncontrolled Keywords:	Amino acid sequence · Bufalo milk · Immunoglobulin G · Mass spectrometry · N-glycosylation
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 02 Amino acids 600 Technology > 08 Food technology > 27 Dairy products
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Feb 2021 04:11
Last Modified:	08 Feb 2021 04:11
URI:	http://ir.cftri.res.in/id/eprint/14783

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