Quantification of aflatoxin and ochratoxin contamination in animal milk using UHPLC-MS/SRM method: a small-scale study.

Rukshan, Mehta and Sweekruthi, A. Shetty and Melissa, F. Young and Barry Ryan, P. and Kannan, Rangiah (2021) Quantification of aflatoxin and ochratoxin contamination in animal milk using UHPLC-MS/SRM method: a small-scale study. Journal of Food Science and Technology. ISSN 0022-1155

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Abstract

Mycotoxin contamination in animal milk is an emerging concern around the globe. Here we developed and validated an ultrahigh-performance liquid chromatography and mass spectrometry-selected reaction monitoring (UHPLC/MS-SRM) method to quantify low concentrations of aflatoxins (AFs) and ochratoxins (OTs) in routinely consumed animal milk samples collected from southern India. Stable isotope dilution methodology was applied to quantify AFB1, AFB2, AFG1, AFG2, AFM1, AFM2 and OTA, OTB in n = 38 different milk samples, using 1 mL of milk. Bioanalytical parameters including method accuracy, precision, recovery, regression analysis and stability were assessed. Dynamic ranges for quantification were between 15.6–1000 pg/mL for AFB1, AFB2, AFG1, and OTA; 7.8–500 pg/mL for AFM1, AFM2 and OTB; 78.6–5000 pg/mL for AFG2. Method accuracy ranged between 80–120%, with ± 15% precision. Recoveries for spiked standards were [ 88% in water and 75% in milk, with limits of quantification (LOQ) ranging between 31.3 pg/mL for AFB1, AFB2, AFG1 and OTA, 15.6 pg/mL for AFM1, AFM2 and OTB and 156 pg/mL for AFG2. R2 values for regression analyses ranged between 0.9991–0.9999. AFB2 [mean: 38 pg/mL (0.038 lg/kg)] was quantified in goat milk, AFM1 was quantified in cow, goat, pasteurized milk [mean: 331 pg/mL (0.331 lg/kg), 406 pg/mL (0.406 lg/kg), 164 pg/mL (0.164 lg/kg)]. Additionally, 90% of cow, goat and pasteurized milk samples were above European Union (EU) limits of 50 pg/ mL (0.05 lg/kg) and 40% of cow and goat milk samples were above the Food Safety Standards Authority of India (FSSAI) limit of 500 pg/mL (0.5 lg/kg). AFM2 was also quantified in cow, goat, and pasteurized milk samples [mean: 249 pg/mL (0.249 lg/kg), 375 pg/mL (0.375 lg/ kg), 81 pg/mL (0.081 lg/kg)]. Our dynamic ranges for quantification are lower than other published methods, with need for a smaller volume of milk. This validated method can be applied for routine quantification of mycotoxins in milk.

Item Type:	Article
Uncontrolled Keywords:	Aflatoxin Ochratoxin Milk UHPLC-MS/ SRM method Quantification
Subjects:	600 Technology > 08 Food technology > 10 Food Microorganisms 600 Technology > 08 Food technology > 27 Dairy products
Divisions:	Food Safety Analytical Quality Control Lab
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Feb 2021 05:58
Last Modified:	08 Feb 2021 05:58
URI:	http://ir.cftri.res.in/id/eprint/14792

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