Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities

Nagaraju, Pramod G and Sengupta, Parineeta and Chicgovinda, Poornima Priyadarshini and Rao, Pooja J (2021) Nanoencapsulation of clove oil and study of physicochemical properties, cytotoxic, hemolytic, and antioxidant activities. Journal of Food Process Engineering. pp. 1-14. ISSN ISSN:1745-4530

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Abstract

The therapeutic properties of clove oil are well known, however, the pungent nature, chemical instability, and low water solubility impose limitations in harnessing its potential. Nanoemulsion of clove oil (CON) was prepared to overcome the above constraints. The non-ionic surfactants Tween 20, 40, and 80 with hydrophile lipophile balance (HLB) of 16.7, 15.6, and 15, respectively, were used to stabilize CON. The creaming index of CON prepared with Tween 20, 40, and 80 was 22.75, 17.5, and 1.5%, respectively, after 8 days of storage at room temperature. Tween20 and 40 produced particles >300 nm while Tween 80 produced �150 nm. Transmission electron microscopic image of spray-dried CON (with Tween 80) showed particles in the range of 150–190 nm. The in vitro release studies showed 76 and 42% cumulative release of CON and native clove oil (NC), respectively, at pH 7.4. The cellular toxicity of CON was significantly reduced by fourfold compared to NC at a concentration of 60 μg/ml when tested on CaCO2 cells. Similarly, hemolytic activity on red blood cells revealed less than 10% hemolysis. In addition, CON also exhibited higher in-vitro antioxidant compared to NC as shown by 1, 1-diphenylpicrylhydrazyl and 2,20-azino-di-[3-ethylbenzthiazoline sulfonate (6)] (ABTS) radical scavenging activity.

Item Type:	Article
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences 500 Natural Sciences and Mathematics > 07 Life Sciences > 01 Biology 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology 600 Technology 600 Technology > 08 Food technology > 07 Food Engineering
Divisions:	Molecular Nutrition
Depositing User:	Mr Pramod G N
Date Deposited:	10 Nov 2021 10:27
Last Modified:	10 Nov 2021 10:27
URI:	http://ir.cftri.res.in/id/eprint/14801

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