Screening and selection of media components for lactic acid production using Plackett-Burman design.

Krishnan, S. and Prapulla, S. G. and Rajalakshmi, D. and Misra, M. C. and Karanth, N. G. (1998) Screening and selection of media components for lactic acid production using Plackett-Burman design. Bioprocess and Biosystems Engineering, 19 (1). pp. 61-65. ISSN 1615-7591

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Abstract

Plackett-Burman design was used to efficiently select important media components influencing lactic acid production in a two step screening procedure. A total of 36 screening experiments were conducted for studying the effect of various media components such as carbon and nitrogen (simple and complex) sources, minerals/buffering agents and a specific inducer for the production of lactic acid by Lactobacillus plantarum NCIM 2084. The eleven ingredients chosen after the first screening experiments were further screened by a Plackett-Burman design consisting of 12 experiments. Liquefied starch, wheat bran extract, ammonium nitrate, manganese sulphate and sodium acetate were chosen as promising ingredients for further optimisation studies. The highest yield of 41.9 g/l of lactic acid was obtained at the end of 24 hours of fermentation which corresponded to 90% conversion, on the basis of sugar supplied.

Item Type:	Article
Additional Information:	Copyright of this article belongs to Springer-Verlag
Subjects:	600 Technology > 08 Food technology > 27 Dairy products
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	08 Oct 2007 12:43
Last Modified:	28 Dec 2011 09:31
URI:	http://ir.cftri.res.in/id/eprint/1494

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