A Spectrophotometric Method for Phosphine Residue Determination in Legumes

Rangaswamy, J. R. and Gunasekaran, N. (1995) A Spectrophotometric Method for Phosphine Residue Determination in Legumes. Journal of Food Science and Technology, 32 (1). pp. 42-45.

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Abstract

A simple spectrophotometric method for the determination of phosphine residue in legumes has been developed. It is based on the reaction of phosphine with silver nitrate in iso-propanol to form a pale yellow chromophore, with an absorption maximum at 436 nm. It gave a linear relationship between the absorbance at 436 nm and the amount of phosphine in the range of 0.02 to 0.48 µg/3 ml reagent. The crop extractives from the legumes showed a low absorbance of 0.008 at 436 nm, and did not interfere in the chromophore reaction. The method is sensitive with determination limit of 0.015 µg phosphine and can be applied for determination of 0.02 µg residue in lOg legume samples. Phosphine residue on legumes, when extracted with a known volume of 2% aqueous isopropanolic silver nitrate, formed egg yellow chromophore with absorption maximum at the same range. Recovery of fortified phosphine from a closed system ranged from 97 to 98%.

Item Type:	Article
Uncontrolled Keywords:	Phosphine residues, Legumes, Phosphine holding capacity, Spectrophotometric method
Subjects:	600 Technology > 08 Food technology > 22 Legumes-Pulses 600 Technology > 03 Agriculture > 05 Insect/Pest Control
Divisions:	Food Protectants and Infestation Control
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	11 Dec 2007 09:24
Last Modified:	30 Dec 2016 11:29
URI:	http://ir.cftri.res.in/id/eprint/1584

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