In vitro‑derived plants grown from low nitrate medium produced quality tubers in Decalepis hamiltonii Wight & Arn.

Umashankar, K. and Pradeep, M. and Giridhar, P. (2022) In vitro‑derived plants grown from low nitrate medium produced quality tubers in Decalepis hamiltonii Wight & Arn. 3 Biotech (2022) :, 12 (200). pp. 1-11.

PDF 3 Biotech (2022) 12 200.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

In the present study, infuence of nitrate concentration on in vitro rooting and its ex vitro hardened tubers were investigated in D. hamiltonii. In vitro multiple shoots established on the MS medium were used as explants for this study and concentration of indole-3-butyric acid (1.23 µM) required for root initiation was determined. The efect of diferent nitrate concentrations in MS medium for in vitro rooting was investigated and positive (¼ and ½ strength) infuence was observed on 14 days inoculation. The in vitro rooted plants (IVP) showed 80% survival upon hardening and exhibited similar growth pattern to seedling plants. The 1-year grew ex vitro hardened plants were evaluated for their tuber quality with reference to their yield and favor metabolite 2-hydroxy-4-methoxy benzaldehyde (2H4MB) content. The IVP grown from ¼ nitrate strength media produced 155±4.85 g FW of tuber biomass which is observed to be higher than full nitrate strength IVP (112±2.52 g) which are grown under similar conditions in the greenhouse. The favor metabolite content, total phenolics, total favonoids, and antioxidant potential of these IVP tubers were evaluated. Upregulation of favor biosynthetic pathway genes DhPAL, DhC4H and DhCoMT were observed in tubers of potted plants that developed from low nitrate strength culture media. In this study, superiority of tissue cultured plants was evident, wherein in vitro plants developed in low nitrate strength medium and acclimatized could produce a higher yield in tuber biomass and maintain relative content of favor compounds in this endangered plant

Item Type:	Article
Uncontrolled Keywords:	Endangered · Endemic · In vitro rooting · Nitrate concentration · 2-Hydroxy-4-methoxybenzaldehyde
Subjects:	500 Natural Sciences and Mathematics > 10 Plants 600 Technology > 08 Food technology > 23 Vegetables > Swallow root
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	24 Jan 2023 08:53
Last Modified:	24 Jan 2023 08:53
URI:	http://ir.cftri.res.in/id/eprint/15846

Actions (login required)

View Item