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Corncob-induced endo-1,4-beta-d-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan.
Ayyappan, Appukuttan Aachary and Prapulla, S. G. (2008) Corncob-induced endo-1,4-beta-d-xylanase of Aspergillus oryzae MTCC 5154: production and characterization of xylobiose from glucuronoxylan. Journal of Agricultural and Food Chemistry, 56 (11). pp. 3981-3988. ISSN 0021-8561
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Abstract
Eight different fungi were cultivated in a peptone-yeast extract medium containing 1% oat spelt xylan (OSX) to evaluate endo-1,4-beta-xylanase secretion for xylooligosaccharide (XOS) production. Aspergillus oryzae MTCC 5154, Aspergillus flavus , Aspergillus niger , and Aspergillus ochraceus showed significant titers of endoxylanases, which were further used for the production of XOS from birch wood xylan (BWX). A. oryzae produced 89.5 +/- 1.13% XOS in the hydrolysate at 24 h of reaction. The effect of OSX, BWX, and raw corncob on the induction of endoxylanase in A. oryzae was studied, and the xylanase activity was maximum at 96 h of cultivation in 3% corncob containing medium. XOS produced at 36 h of reaction was 5.87 +/- 0.53 mg/mL (12 +/- 2% xylose, 48 +/- 2.43% xylobiose, and 40 +/- 3.6% higher oligomers) from 1% BWX . HPLC/refractive index detection and ESI/MS analysis of fractions obtained by GPC corresponded to neutral and 4- O-methyl-alpha- d-glucuronic acid substituted acidic oligosaccharides. The major fraction, beta- d-xylopyranosyl-(1-->4)- d-xylanopyranose was characterized using (13)C NMR.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Aspergillus oryzae MTCC 5154; endo-1,4-�-xylanase; corncob; xylooligosaccharides; xylobiose; 13C NMR |
| Subjects: | 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi |
| Divisions: | Fermentation Technology and Bioengineering |
| Depositing User: | Food Sci. & Technol. Information Services |
| Date Deposited: | 08 Aug 2008 10:34 |
| Last Modified: | 21 Jun 2012 06:20 |
| URI: | http://ir.cftri.res.in/id/eprint/1785 |
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