Physicochemical comparison of chitin characteristics in three major stored-product beetle pests: Implications for biofumigant toxicity.

Pratiksha Prabhakar, Gawali and Veeresh, T. and Madhurya, Lokesh and Sudheer, K. Yannam and Ezhilvendan, S. (2024) Physicochemical comparison of chitin characteristics in three major stored-product beetle pests: Implications for biofumigant toxicity. International Journal of Biological Macromolecules, 265. p. 130759.

PDF 1-s2.0-S0141813024015630-main.pdf - Published Version Restricted to Registered users only Download (4MB) | Request a copy

Abstract

The present study investigates the chitin properties of stored-product insect pests and their association with the fumigant toxicity of garlic essential oil. Chitin isolates of Callosobruchus maculatus, Sitophilus oryzae, and Tribolium castaneum adults were characterized using FT-IR, XRD, EA, SEM-EDS, and NMR techniques. Fumigant toxicity assay was performed under airtight condition in glass vial. The S. oryzae contains highest chitin content (19 %), followed by T. castaneum (10 %) and C. maculatus (8 %). The degree of crystallinity was lower in C. maculatus (67.13 %) than in S. oryzae (77.05 %) and T. castaneum (76.56 %). Morphologically, C. maculatus chitin displayed a flat lamellar surface with pores, while S. oryzae and T. castaneum exhibited densely arranged microfibrils based surfaces. Fumigant toxicity assays revealed varied susceptibility levels, C. maculatus exhibited higher susceptibility (0.27 μL/L air of LC50) compared to S. oryzae and T. castaneum (14.35 and 3.74 μL/L air of LC50, respectively) to garlic essential oil. The higher chitin content, greater crystallinity, and densely arranged structures in S. oryzae might contribute to its tolerance towards fumigant. Additionally, physico-chemical properties and penetration potentiality of the bioactive constituents might be linked to the toxicity in insects. Understanding these relations can enrich knowledge of chitin's role in fumigant toxicity mechanism.

Item Type:	Article
Uncontrolled Keywords:	Chitin Callosobruchus maculatus Sitophilus oryzae Tribolium castaneum Fumigant toxicity Phytochemical volatiles
Subjects:	600 Technology > 03 Agriculture > 05 Insect/Pest Control
Divisions:	Dept. of Biochemistry Food Protectants and Infestation Control Lipid Science and Traditional Foods
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	06 Nov 2024 07:11
Last Modified:	06 Nov 2024 07:11
URI:	http://ir.cftri.res.in/id/eprint/18398

Actions (login required)

View Item