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Regulation of astaxanthin and its intermediates through cloning and genetic transformation of Beta-carotene ketolase in Haematococcus pluvialis
Kathiresan, S. and Arun, Chandrashekar and Ravishankar, G. A. and Sarada, R. (2015) Regulation of astaxanthin and its intermediates through cloning and genetic transformation of Beta-carotene ketolase in Haematococcus pluvialis. Journal of Biotechnology, 196 (197). pp. 33-41.
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Abstract
Astaxanthin, a high-value ketocarotenoid used in the pharmaceutical and nutraceutical industries is mainly produced from green alga, Haematococcus pluvialis. It is biosynthesized by the action of key enzyme, -carotene ketolase (BKT) on -carotene through intermediates echinenone and canthaxan- thin. In this study, the -carotene ketolase (bkt) gene was isolated from H. pluvialis and cloned in a vector pRT100 and further mobilized to a binary vector pCAMBIA 1304. The T-DNA of pCAMBIA 1304, which consists of cloned bkt, was successfully transformed to H. pluvialis through Agrobacterium mediation. The cloning and transformation of bkt in H. pluvialis was confirmed by Southern blotting and also by PCR analysis. Total carotenoids and astaxanthin content in the transformed cells were found to be 2–3-fold higher, while the intermediates like echinenone and canthaxanthin were found to be 8–10-fold higher than in the control cells. The expression level of carotenogenic genes like phytoene synthase (psy), phy- toene desaturase (pds), lycopene cyclase (lcy), bkt, and -carotene hydroxylase (bkh) were found to be higher in transformed cells compared to the non-transformed (NT) H. pluvialis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Haematococcus pluvialis, Beta carotene ketolase, Astaxanthin, Agrobacterium, Transformation |
| Subjects: | 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry 600 Technology > 08 Food technology > 29 Microbiological food > 01 Algae |
| Divisions: | Plant Cell Biotechnology |
| Depositing User: | Somashekar K S |
| Date Deposited: | 10 Mar 2025 09:04 |
| Last Modified: | 10 Mar 2025 09:04 |
| URI: | http://ir.cftri.res.in/id/eprint/19250 |
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