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Protective effect of phytochemicals-rich Syzygium malaccense seed extract against H2O2 stimulated inflammatory response in RAW 264.7 macrophages
Manisha, Chauhan and Pradeep Singh, Negi (2025) Protective effect of phytochemicals-rich Syzygium malaccense seed extract against H2O2 stimulated inflammatory response in RAW 264.7 macrophages. Journal of Food Bioscience, 66. pp. 1-10.
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Abstract
Fruit seeds contain various phytochemicals, including polyphenols, tannins, and carotenoids, with exceptional antioxidant properties. This study aimed to extract polyphenols from green wax apple seeds and study their anti- oxidant and anti-inflammatory properties in an in-vitro inflammatory model. The extracts showed potent anti oxidant activities in ABTS, DPPH, NOS, and FRAP assays. The UPLC analysis revealed various phenolic com pounds (Vanillic acid, protocatechuic acid, syringic acid, ellagic acid, and ECG), and ultrasound-assisted extraction was found to be superior due to its higher extraction efficiency. In in-vitro assays in RAW264.7 cells, the aqueous UAE extract inhibited the NF-ĸB pathway by reducing the production of pro-inflammatory cytokines against H2O2-induced inflammation. The chlorogenic acid, vanillic acid, and syringic acid strongly correlated with ABTS activity, and epicatechin gallate and vanillic acid correlated with antipathogenic activity. The high anti-oxidant, antipathogenic, and anti-inflammatory activities in the seed of Syzygium malaccense indicate its potential for utilization in functional foods.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Polyphenols, Ultrasound assisted extraction, Steam explosion, Anti-inflammatory effects, Anti-pathogenic activity, ROS scavenging activity, Syzygium malaccense |
| Subjects: | 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 18 Phytochemistry 600 Technology > 08 Food technology > 24 Fruits |
| Divisions: | Fruit and Vegetable Technology |
| Depositing User: | Somashekar K S |
| Date Deposited: | 24 Jun 2025 09:23 |
| Last Modified: | 24 Jun 2025 09:23 |
| URI: | http://ir.cftri.res.in/id/eprint/19571 |
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