Chia seed mucilage free oligosaccharides: Evaluation of Lactiplantibacillus plantarum WCFS1 α-galactosidase (LpαG) activities and prebiotic effect

Lijina, Pannikal and Ritesh, Ranjan and Mukesh, Kapoor and Gnanesh Kumar Belur, Shivappa (2025) Chia seed mucilage free oligosaccharides: Evaluation of Lactiplantibacillus plantarum WCFS1 α-galactosidase (LpαG) activities and prebiotic effect. Journal of Food Bioscience, 68. pp. 1-9.

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Abstract

Chia seed mucilage was probed for the presence of structurally diverse free oligosaccharides along with the assessment of hydrolytic and transgalactosylation ac­ tivities of α-galactosidase from a probiotic bacterium- Lactiplantibacillus plantarum WCFS1 (LpαG) on isolated oligosaccharides. Porous graphitic carbon-liquid chromatography-tandem mass spectrometry (PGC LC-MS/MS) analysis revealed the presence of mainly sucrose (disaccharide), planteose (trisaccharide), few isomeric tetrasaccharides as well as lower abundance of isomeric tri-, tetra-, penta- and hexasaccharides. In vitro evaluation of the digestibility of planteose and tetrasaccharides to the intestinal enzymes did not generate monosaccharides. However, LpαG displayed hydrolytic activity towards planteose and selectively acted on two tetrasaccharides, which were identified as sesamose and stachyose. In addition, transglycosylation property of LpαG using planteose led to the synthesis of sesamose. Thus, the hydrolysis of planteose and sesamose was demonstrated for the first time for an α-galactosidase from the probiotic bacterium. Furthermore, the oligosaccharides pool was shown to influence the growth of probiotic L. acidophilus, L. brevis and L. plantarum in vitro, along with the production of short chain fatty acids (SCFAs). These findings would contribute to the potential application of chia seeds to further enhance its health benefits.

Item Type:	Article
Uncontrolled Keywords:	Chia seed mucilage, oligosaccharides, Lactiplantibacillus plantarum WCFS1
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 07 Enzyme Biochemistry 600 Technology > 08 Food technology > 16 Nutritive value > 08 Grains
Divisions:	Dept. of Biochemistry Food Microbiology
Depositing User:	Somashekar K S
Date Deposited:	24 Jun 2025 10:42
Last Modified:	24 Jun 2025 10:42
URI:	http://ir.cftri.res.in/id/eprint/19575

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