Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide.

Gulla, K. C. and Gouda, M. D. and Thakur, M. S. and Karanth, N. G. (2002) Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide. Biochimica et Biophysica Acta, 1597 (1). pp. 133-9. ISSN 0006-3002

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Abstract

Biosensors based on acetyl cholinesterase (AChE) inhibition have been known for monitoring of pesticides in food and water samples. However, strong inhibition of the enzyme is a major drawback in practical application of the biosensor which can be overcome by reactivation of the enzyme for repeated use. In the present study, enzyme reactivation by oximes was explored for this purpose. Two oximes viz., 1,1'-trimethylene bis 4-formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were compared for the reactivation of the immobilized AChE. TMB-4 was found to be a more efficient reactivator under repeated use, retaining more than 60% of initial activity after 11 reuses, whereas in the case of 2-PAM, the activity retention dropped to less than 50% after only 6 reuses. Investigations also showed that reactivation must be effected within 10 min after each analysis to eliminate the ageing effect, which reduces the efficiency of reactivation.

Item Type:	Article
Uncontrolled Keywords:	Organophosphorus pesticide; Biosensor; Acetyl cholinesterase; Oxime
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	16 Feb 2018 05:49
Last Modified:	16 Feb 2018 05:49
URI:	http://ir.cftri.res.in/id/eprint/2107

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