In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents

Obul Reddy, B. and Giridhar, P. and Ravishankar, G. A. (2001) In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root-promoting agents. Current Science, 81 (11). pp. 1479-1482.

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Abstract

In the present study micropropagation of an endangered shrub, Decalepis hamiltonii Wight & Arn., optimization and standardization of efficient methods for rooting of in vitro-derived micro-shoots by using phloroglucinol (PG), activated charcoal and CoCl2 have been achieved. Transfer of in vitro-derived shoots to MS medium supplemented with 8.8 mM indole-3- butyric acid (IBA) and 1.43 mM indole-3-acetic acid resulted in root induction. In another treatment, IBA was found to be a good root-promoting agent, wherein dipping of explants in 4.4 μM IBA for 30 min and subsequent inoculation on MS basal medium was also beneficial for root induction. Successful field transfer was accomplished in rooted plantlets.

Item Type:	Article
Uncontrolled Keywords:	Decalepis hamiltonii, culinary spice, aromatic roots, micropropagation protocols
Subjects:	500 Natural Sciences and Mathematics > 10 Plants > 06 Trees And Shrubs 500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions:	Plant Cell Biotechnology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Jan 2009 09:43
Last Modified:	28 Dec 2011 09:38
URI:	http://ir.cftri.res.in/id/eprint/2118

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