Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels.

Suresh Babu, C. V. and Dubey, A. K. and Kini, R. and Umesh Kumar, S. and Karanth, N. G. (1999) Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels. Electrophoresis, 20 (3). pp. 483-485. ISSN 0173-0835

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Abstract

A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium chloride is described. The reagent reacts with the reducing group of glucose released by glucoamylase from the substrate starch. Using the reaction, production of glucoamylase by Aspergillus niger was detected on 10% IEF gels within a pH range of 2.5-9.5. Since the method can detect raw and gelatinized starch activities of glucomylase associated with 1 microg protein, it will be useful for enzyme engineering studies that involve screening of various mutations.

Item Type:	Article
Uncontrolled Keywords:	Isoelectric focusing Glucoamylase Raw starch hydrolysis Direct detection
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry 600 Technology > 08 Food technology > 25 Sugar/Starch/Confectionery
Divisions:	Fermentation Technology and Bioengineering Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	14 Feb 2012 05:58
Last Modified:	30 May 2012 11:11
URI:	http://ir.cftri.res.in/id/eprint/2198

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