Determination of molecular weight and related hydrodynamic parameters of high molecular weight protein fraction from a few oilseeds.

Murty, B. S. and Prakash, V. and Rao, M. S. N. (1990) Determination of molecular weight and related hydrodynamic parameters of high molecular weight protein fraction from a few oilseeds. Indian journal of biochemistry & biophysics, 27 (5). pp. 295-9. ISSN 0301-1208

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Abstract

The hydrodynamic parameters of the major protein fraction, viz. arachin from groundnut, alpha-globulin from sesame seed, brassin (M) from mustard seed and helianthinin from sunflower seed, have been determined in a single solvent system (0.05 M Tris-HCl buffer, pH 7.5 containing 0.5 M sodium chloride): sedimentation coefficient (s0(20,w)) and diffusion coefficient (D0(20,w)) by analytical ultracentrifugation, intrinsic viscosity [eta] by Ostwald viscometry and partial specific volume (V) by densimetry. The molecular weights (M) of the four proteins, calculated using the sedimentation-viscosity and sedimentation-diffusion coefficient methods, were found close to each other. The values have been compared with those in the literature and the reasons for discrepancies have been discussed.

Item Type:	Article
Uncontrolled Keywords:	oilseeds, molecular weight, protein fractions
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins 600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	15 Nov 2021 06:01
Last Modified:	15 Nov 2021 06:01
URI:	http://ir.cftri.res.in/id/eprint/2343

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