Biotransformation of terpenic substrates by resting cells of Aspergillus niger and Pseudomonas putida isolates

Divyashree, M. S. and George, J. and Agrawal, R. (2005) Biotransformation of terpenic substrates by resting cells of Aspergillus niger and Pseudomonas putida isolates. Journal of Food Science and Technology (India), 43 (1). pp. 73-76.

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Abstract

<p align="justify">Readily available and low cost terpenic compounds like (+) limonene, (-) limonene, (+) α-pinene, (-) α-pinene and (-) β-pinene, were used as substrates for biotransformation by a local fungal isolate of Aspergillus niger and a bacterial strain of Pseudomona putida. The cultures were grown in potato dextrose broth at 30°C to their exponential phase and harvested. The resting cells were taken in phosphate buffer (0.05M; pH 7.0). Each substrate was added separately (2 mg/25 ml buffer) and incubated at 30°C for 6 h, after the reaction the flavouring compounds were extracted in dichloromethane, dried over anhydrous sodium sulphate, identified and analysed by GCMS. Aspergillus niger could use (-) limonene as the substrate to give carveol (18%) and dihydrocarveol (15%). Bioconversion from (+) a-pinene was better with Pseudomonas putida, producing industrially important compounds like verbenol (35%), dihydrocarveol acetate (20%) and verbenone (10%) in high yields. However, the bacteria was unable to bioconvert (-) α-pinene, (-) β-pinene, and (-) (+) limonenes to these important compounds.</p>

Item Type:	Article
Uncontrolled Keywords:	Aspergillus niger, Pseudomonas putida, Pinene, Limonene, Chiral, Biotransformation
Subjects:	500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi 600 Technology > 08 Food technology > 09 Food Microbiology 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 33 Terpenoids Chemistry
Divisions:	Food Microbiology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	29 Dec 2005
Last Modified:	11 May 2012 04:05
URI:	http://ir.cftri.res.in/id/eprint/282

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