Biosensor for the determination of phenols based on cross-linked enzyme crystals (CLEC) of laccase.

Jegan Roy, J. and Abraham, T. E. and Abhijith, K. S. and Sujith Kumar, P. V. and Thakur, M. S. (2005) Biosensor for the determination of phenols based on cross-linked enzyme crystals (CLEC) of laccase. Biosensors and Bioelectronics, 21 (1). pp. 206-211.

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Abstract

Cross-linked enzyme crystals (CLECs) are a versatile form of biocatalyst that can also be used for biosensor application. Laccase from Trametes versicolor (E.C.1.10.3.2) was crystallized, cross-linked and lyophilized with �-cyclodextrin. The CLEC laccase was found to be highly active towards phenols like 2-amino phenol, guaiacol, catechol, pyrogallol, catechin and ABTS (non-phenolic). The CLEC laccase was embedded in 30% polyvinylpropylidone (PVP) gel and mounted into an electrode to make the sensor. The biosensor was used to detect the phenols in 50–1000 �mol concentration level. Phenols with lower molecular weight such as 2-amino phenol, catechol and pyrogallol gave a short response time where as the higher molecular weight substrates like catechin and ABTS had comparatively a long response time. The optimum pH of the analyte was 5.5–6.0 when catechol was used as substrate. The CLEC laccase retained good activity for over 3 months.

Item Type:	Article
Uncontrolled Keywords:	Glutaraldehyde; Phenolic compounds
Subjects:	600 Technology > 08 Food technology > 07 Food Engineering 600 Technology > 08 Food technology > 11 Food Biochemistry
Divisions:	Fermentation Technology and Bioengineering
Depositing User:	Users 197 not found.
Date Deposited:	20 Jul 2011 09:34
Last Modified:	28 Dec 2011 09:43
URI:	http://ir.cftri.res.in/id/eprint/3191

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