Stabilization of cetyltrimethyl ammonium bromide permeabilized yeast whole cell lactase.

Joshi, M. S. and Bachhawat, N. and Bhat, S. G. (1989) Stabilization of cetyltrimethyl ammonium bromide permeabilized yeast whole cell lactase. Biotechnology Letters, 11 (5). pp. 349-352.

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Abstract

Cetyltrimethylammonium bromide-permeabilized cells ofK. fragilis loose ~-galactosidase activity due to leaking of the enzyme into the medium. This leakage of the enzyme can be prevented by storing the permeabilized cells either in buffer containing 50,% glycerol or by treating the permeabilized cells with 0.2,% glutaraldehyde at 4~ for 10 min. In repeated batch hydrolysis of lactose in milk, glutaraldehyde treated cells could be repeatedly used very efficiently.

Item Type:	Article
Uncontrolled Keywords:	beta-galactosidase, lactase, Kluyveromyces fragilis, Cetyltrimethylammonium bromide
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes 600 Technology > 08 Food technology > 29 Microbiological food > 04 Yeast
Divisions:	Dept. of Biochemistry
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	05 Mar 2018 08:58
Last Modified:	05 Mar 2018 08:58
URI:	http://ir.cftri.res.in/id/eprint/5473

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