Reverse phase liquid chromatographic determination of some food additives.

Veerabhadrarao, M. and Narayan, M. S. and Kapur, O. and Sastry, C. S. (1987) Reverse phase liquid chromatographic determination of some food additives. Journal of the Association of Official Analytical Chemists, 70 (3). 578-582, 24 ref..

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Abstract

Liquid chromatographic methods are described for separation and detn. of non-nutritive sweeteners, namely, acesulfame, aspartame, saccharin, and dulcin; preservatives such as benzoic acid and p-hydroxybenzoic acid; and caffeine and vanillin in ready-to-serve beverages, ice candy, ice cream, squash beverage, tomato sauce, and dry beverage mix samples. These additives are separated on a muBondapak C18 column using methanol/acetic acid/water (20 + 5 + 75) as mobile phase and detected by UV absorption at 254 nm. Caffeine, vanillin, dulcin, and benzoic acid can be analysed quickly by using a mobile phase of methanol/acetic acid/water (35 + 5 + 60). Aspartame can be separated in the presence of caffeine and vanillin by using the mobile phase pH 3 acetate buffer/methanol (95 + 5). Retention factors and min. detectable limits are described. The % error and the % relative s.d. for 6 replicate samples ranged from 0.3 to 2.8 and from 1.64 to 3.60, resp. Recovery of additives added to the foods named and analysed by the direct method and by extraction ranged from 98.0 to 100.6% and from 91.6 to 101.8%, resp. The proposed LC techniques are simple, rapid, and advantageous because all the additives can be detected in a single step, which makes it useful for routine analysis of various food products.

Item Type:	Article
Uncontrolled Keywords:	ADDITIVES-; foods, additives liquid chromatographic detn. in; CHROMATOGRAPHY-; SWEETENERS-; foods, sweeteners liquid chromatographic detn. in; PRESERVATIVES-; foods, preservatives liquid chromatographic detn. in
Subjects:	500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography 600 Technology > 08 Food technology > 31 Food Additives
Divisions:	Food Safety Analytical Quality Control Lab
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	23 Aug 2021 04:48
Last Modified:	23 Aug 2021 04:48
URI:	http://ir.cftri.res.in/id/eprint/6434

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