Relationships between grain hardness and the contents of prolamin and three anti-fungal proteins in sorghum.

Kumari, S. R. and Chandrashekar, A. (1994) Relationships between grain hardness and the contents of prolamin and three anti-fungal proteins in sorghum. Journal of Cereal Science, 20 (1). 93-99, 21 ref..

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Abstract

Relationship between variation in prolamin content and variation in the contents of 3 antifungal proteins (mol. wt. 18, 26, and 30 kDa) was studied in normal sorghum lines using ELISA. Since a prolamin gradient exists within sorghum grains, these differences were related to those of the 3 antifungal proteins in the sorghum endosperm using immunofluorescence microscopy. 40 cv. of sorghum were used that varied in grain hardness and prolamin content. Significant relationships were observed between contents of antifungal proteins and prolamin content. The antifungal proteins were found to be present at a higher level in the corneous endosperm than in the floury endosperm. From results using immunofluorescence microscopy, the 18 kDa protein was concentrated along the cell walls where the protein bodies are located. The 26 kDa protein was associated primarily with the cell walls and not with the protein bodies. No fluorescence was observed for the 30 kDa protein. It is concluded that a locational and quantitative link between the sorghum prolamins and antifungal proteins exists.

Item Type:	Article
Uncontrolled Keywords:	CEREALS-; PROTEINS-; PROTEINS-CEREAL; SORGHUM-; PROLAMINS-
Subjects:	600 Technology > 08 Food technology > 21 Cereals > 06 Sorghum 600 Technology > 08 Food technology > 09 Food Microbiology
Divisions:	Food Microbiology Grain Science and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	14 Mar 2012 08:49
Last Modified:	14 Mar 2012 08:49
URI:	http://ir.cftri.res.in/id/eprint/6526

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