Fractionation of leaf proteins of lucerne (Medicago sativa) using organic solvents.

Gurumukh, Singh. and Narendra, Singh. (1985) Fractionation of leaf proteins of lucerne (Medicago sativa) using organic solvents. Journal of Food Science and Technology, 22 (1). 69-70, 8 ref..

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Abstract

Proteins from lucerne juice were fractionated using iso-amyl alcohol, n-butanol, iso-butanol, ethyl acetate, diethyl ether, iso-propanol, ethanol and acetone, with % concn. ranging from 4 to 26 (v/v). Optimum pH for fractionation was 6.0-6.4. Yield of cytoplasmic leaf protein concentrate (LPC) decreased with increase in solvent level; with isoamyl alcohol, cytoplasmic LPC yield was max. (1.63 g/100 ml extract) at 10:0.40 (v/v) concn., whereas with ethyl acetate max. yield (1.51 g/100 ml extract) was at 10:0.75 (v/v) concn. Those solvents having limited water solubility brought more protein into the cytoplasmic fraction than water miscible solvents. Cytoplasmic protein concentrates did not have a green colour.

Item Type:	Article
Uncontrolled Keywords:	PROTEIN-PRODUCTS; lucerne leaf proteins, solvents fractionation of; SOLVENTS-; FRACTIONATION-; LUCERNE-; solvents fractionation of lucerne leaf proteins
Subjects:	600 Technology > 08 Food technology > 16 Nutritive value > 09 Leaf protein
Divisions:	Protein Chemistry and Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	01 Oct 2010 04:27
Last Modified:	28 Dec 2011 09:54
URI:	http://ir.cftri.res.in/id/eprint/6576

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