Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit

Anand, P. Kulkarni and Aradhya, S. M. and Soundar, Divakar (2004) Isolation and identification of a radical scavenging antioxidant – punicalagin from pith and carpellary membrane of pomegranate fruit. Food Chemistry, 87. pp. 551-557.

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Abstract

Pith and carpellary membrane (CM) constitute 13% of pomegranate fruit composition. Pith acts as an anchoring tissue while CM acts as a protective membrane; both play significant roles in determining the health and disease status of pomegranate arils but remain unexplored for their nutraceutical properties. Activity-guided repeated fractionation of the methanol extract on a silica gel column, followed by preparative HPLC, yielded a compound which exhibited strong radical scavenging activity with EC50 values of 16.7�2.3 and 54.2�4.6 against DPPH free radical and superoxide radicals, respectively. It also showed a very strong lipid peroxidation inhibitory activity in a liposome model system with an EC50 value of 54.2�0.9. However, it showed negligible metal chelating activity. Based on UV, IR, 2D-NMR, GC-Mass and MALDI-Mass studies, the compound was identified as punicalagin. The antioxidant activity of punicalagin and the methanol extract can be ascribed mainly to radical scavenging ability.

Item Type:	Article
Uncontrolled Keywords:	Punica granatum; Antioxidant; Isolation; Identification; Pith and carpellary membrane; Punicalagin
Subjects:	600 Technology > 08 Food technology > 24 Fruits 600 Technology > 08 Food technology > 32 Antioxidants
Divisions:	Fermentation Technology and Bioengineering Fruit and Vegetable Technology
Depositing User:	Food Sci. & Technol. Information Services
Date Deposited:	26 Nov 2008 11:48
Last Modified:	28 Dec 2011 09:59
URI:	http://ir.cftri.res.in/id/eprint/7576

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